Journal: Frontiers in Aging Neuroscience

Article Title: Ketogenic interventions enhance REM sleep in females and support memory in aged rats

doi: 10.3389/fnagi.2026.1797686

Figure Lengend Snippet: Effect of ketogenic diet intervention on oxidative stress and neuroinflammation in aged rats. (A) Representative immunofluorescence images showing β -III tubulin for neurons (red), Iba1 for microglia (cyan), GFAP for astrocytes (blue), and 4HNE (green) of the hippocampal region from coronal brain sections (50 μm) of aged male and female rats. The fifth column shows an enlarged merged image of the CA1 region of the hippocampus for the indicated groups. All images were acquired at 20X magnification and 3×2 tiles. Graphs showing the average intensity of 4HNE in neurons (B) , microglia (C) , and astrocytes (D) , respectively, quantified using the Ilastik-QuPath workflow for calculating the mean 4HNE intensity in each cell type expressed as arbitrary units (A.U.). Graphs showing the integrated density (Int. Den.) per mm 2 expressed as arbitrary units (A.U.) (E) and no. of 4HNE puncta per mm 2 (F) quantified using NIH-ImageJ software. (G) Representative immunofluorescence images showing β-III tubulin (red), Iba1 (cyan), and TREM2 (green) of the hippocampal region from coronal brain sections (50 μm) of aged male and female rats. The fourth column shows an enlarged merged image of the CA1 region of the hippocampus for the indicated groups. Graphs of mean TREM2 intensity in neurons (H) and microglia (I) , respectively quantified using the Ilastik-QuPath workflow for quantification of mean TREM2 intensity in each cell type expressed as A.U. (J) TREM2 integrated density per mm2 expressed as arbitrary units (A.U.) quantified using NIH-ImageJ software. Individual data points represent data from the CA1 region of the hippocampus from one brain tissue section; three brain sections were imaged per rat and approximately 100–200 cells were analyzed per cell type. The number of detections for each individual cell type for each marker is given in , and the number of rats used for the analysis are given in . Data represent Mean ± SEM. Mixed-effects two-way ANOVA (sex × diet) with animal as a random factor (fields nested within animals) and Tukey post hoc test. * p < 00.05, ** p < 0.01. Control diet (CD) and ketogenic diet (KD).

Article Snippet: The following primary antibodies were used: rabbit anti- 4-hydroxynonenal (4HNE) (1:500; Bioss bs-6313R), rabbit anti- 3-nitrotyrosine (3-NT) (1:200; Bioss BS-8551R), rabbit anti- triggering receptor expressed on myeloid cells 2 (TREM2) (1:200; Bioss BS-2723R), rabbit anti- NLR family pyrin domain containing 3 (NLRP3) (1:100; NBP2-12446), mouse anti-beta- III tubulin (1:400; Abcam AB78078), rat anti- ionized calcium-binding adaptor molecule 1 (IBA) (1:1000; Abcam AB283346 ), chicken anti- glial fibrillary acidic protein (GFAP) (1:1000; Abcam AB4674).

Techniques: Immunofluorescence, Software, Marker, Control

Journal: Hypertension

Article Title: l-Arginine Depletion in Preeclampsia Orients Nitric Oxide Synthase Toward Oxidant Species

doi: 10.1161/01.hyp.0000116220.39793.c9

Figure Lengend Snippet: Figure 2. Tissue localization of lipid per- oxidation products by immunostaining with anti-4-hydroxynonenal-(4-HNE)- lysine Ab. 4-HNE-lysine staining (dark brown) is mostly faint in NP villi (A) and decidua (B). In preeclamptic placenta, intense 4-HNE-lysine staining is evident in syncytiotrophoblast and in the villous endothelium (C and E). F, Immunostain- ing with anti-nitrotyrosine Ab of villous tissue from the same preeclamptic woman, showing colocalization of nitro- tyrosine and 4-HNE-lysine staining. Mod- erate staining is also focally evident on vessels of preeclamptic decidua (D). No signal is found in the negative control (G). v, indicates villous tissue; d, decidua; closed arrow, syncytiotropho- blast; open arrow, endothelium. Original magnifications: A, B, C, D, and G: 200; E and F: 400.

Article Snippet: For immunoperoxidase, mouse monoclonal antibodies against the following antigens: human-ecNOS (Transduction Laboratories, Exeter, UK),1 nitrotyrosine (Upstate Biotechnology Inc, Lake Placid, NY),2 human-CuZn-SOD (Sigma Chemicals Co, St Louis, MO) and 4-hydroxynonenal-(4-HNE)-lysine adduct (NA59, kindly provided by Dr Witzum, The Scripps Research Institute, La Jolla, CA),3 and a rabbit polyclonal antibody against human-arginase II (Santa Cruz Biotechnology, Inc., USA)4 were used.

Techniques: Immunostaining, Staining, Negative Control

Journal: European Journal of Histochemistry : EJH

Article Title: Ketogenic diet regulates Uch-L1(C) to improve cerebral energy metabolism and cognitive function in Alzheimer's disease mice

doi: 10.4081/ejh.2026.4548

Figure Lengend Snippet: Detection of oxidative stress in the hippocampus and cortex of mice brain. A,B ) Expression of 8-OHdG and 4-HNE in hippocampus and temporal cortex in different groups examined by immunofluorescence images (n=3). C,D ) Analysis of fluorescence intensity of 8-OHdG in hippocampus and temporal cortex respectively. E,F ) Analysis of fluorescence intensity of 4HNE in hippocampus and temporal cortex, respectively. n=3, * p <0.05, ** p <0.01, *** p <0.001.

Article Snippet: 4-HNE , Stressmarq , SMC-511D , 1:200 (IF) 1:1000 (WB).

Techniques: Expressing, Immunofluorescence, Fluorescence